AG Fiedler
Contact
PD Dr. rer. nat. Tomas Fiedler
location
Anfahrt zum IMIKRO

Main Topics
Systems biology and metabolism of gram-positive cocci
In cooperation with modeller groups we aim to understand the metabolic processes enabling gram-positive homofermentative lactic acid bacteria such as Streptococcus pyogenes and Enterococcus faecalis to colonize (or invade) the human host.
We experimentally support the modelling groups in reconstructing the metabolic networks of those bacteria and construct genome-scale metabolic models considering metabolic, transcriptomic and proteomic data. Based on the models, we try to identify potential new drug targets and develop antimicrobial drugs interfering with metabolic processes specific and vital for the bacteria.
Funding
DFG (FI1588/2-1) „Elucidating the role of protein load for the physiology of Enterococcus faecalis by integrating proteomics data and genome-scale computational models” 10/2018-09/2021
Landesgraduiertenförderung M-V: „Die Rolle der nicht-phosphorylierenden Glycerinaldehyd-3-Phosphat-Dehydrogenase GapN im Stoffwechsel von Streptokokken und ihr Potential als drug target in Streptococcus pyogenes“ 10/2020-09/2022
Interactions between bacterial pathogens and biomaterials
SYLOBIO
Systemic and local reactions in case of incompatibility to biomaterials for joint and skin lesions
Research Training Group 2901 SYLOBIO
Project description
The Research Training Group 2901 SYLOBIO funded by the German Research Foundation (DFG), analyses the local as well as the systemic response to biomaterials. The central research question is whether the systemic reaction to biomaterials is either triggered by biological factors from the biomaterial environment or whether systemic exposure to degradation products leads to inflammation in organs/tissues distant from the biomaterial.
In order to identify the complexity of material-induced inflammation and the patient-specific diversity of tissue response, a multidisciplinary approach is required to analyze systemic and local mechanisms of action in detail.
One of the subprojects is located at the Institute of Medical Microbiology, Virology and Hygiene and aims to investigate the impact of wear and corrosion products of metallic prosthetic joints on
- formation and composition of biofilms, and
- antibiotic sensitivity
of Staphylococcus epidermidis and Staphylococcus aureus, the most frequent causes for prosthetic joint infections. Furthermore, the primary impact of wear and corrosion products on antimicrobial activity of phagocytes (neutrophils) as well as the secondary impact via potential immune modulatory effects on mesenchymal stem cells, endothelial cells, and osteoblasts will be investigated within this subproject.
Principal Investigator
Project Team
MSc Ann Charloth Kuhfahl
BSc Ben Hlawatschke
Associated researcher
Funding Period
01.07.2024-30.06.2028
Funding

Microbial enzymes in cancer therapy
Microbial enzymes converting nutrients essential for growth of tumor cells are promising candidates in cancer therapy.
In collaboration with Dr. Claudia Maletzki (Molecular Oncology and Immune Therapy group of the Rostock University Medical Centre) we work on the application of arginine-famishment of solid tumors using bacterial arginine-degrading enzymes.
Group
AG Fiedler 2024

Archiv Gruppenbilder

AG Fiedler 2024

AG Fiedler 2019

PD Dr. rer. nat.
Tomas Fiedler
- Wissenschaftler
am Institut für Medizinische Mikrobiologie, Virologie und Hygiene

Dipl. Ing. (FH)
Yvonne Humboldt
- Medizinisch-technische Assistentin in der Forschung
- Sicherheitsbeauftragte am IMIKRO
- Mitarbeiterin Kurslabor
+49 (0) 381 494 5957
+49 (0) 381 494 5795
+49 (0) 381 494 5902
yvonne.humboldt{bei}med.uni-rostock.de

Isabell Wagner
- PhD Student
am Institut für Medizinische Mikrobiologie, Virologie und Hygiene
+49 (0) 381 494 5939
+49 (0) 381 494 5977
+49 (0) 381 494 5902

Ann Charloth Kuhfahl
- PhD Student
am Institut für Medizinische Mikrobiologie, Virologie und Hygiene
+49 (0) 381 494 5939
+49 (0) 381 494 5977
+49 (0) 381 494 5902
Ben Hlawatschke | Bachelor student | +49 (0) 381 494 5977 |
Inga Kilian | Master student | +49 (0) 381 494 5977 |